Paramecia

Culturing Paramecium multimicronucleatum to feed Chaos

Carolina 130871 Paramecium multimicronucleatum Demoslide Set $13.50

Make medium from Carolina concentrate 132350 Liquid Protozoan Nutrient Concentrate, Culture Medium, Makes 1 gal, 100 mL $11.00

1 mL LPNC, 9 mL sterile water, 1 sterile wheat seed in a 25 mm2 vented culture flask.

1 mL P. m. culture.

20C in the dark.

Check concentration.

• 10 10µL drops of culture
• on a clean (wipe it first!) slide
• count with dissecting scope and side illumination.

Initial concentration ~40/mL

Plot growth.

Scale up.