LB agar calculators
90 plates (3 plates/pair + plates to start cultures from frozen stocks)
25 mL/plate (=~2250 mL LB)
25 g LB + 15 g agar /liter
Make 3 bottles of 750 mL each:
56.25 g LB
2.25 L water
stir (takes a while)
autoclave 45 min (liquid cycle)
put 750 mL in each of 3 1 L media bottles
add a stir bar
add 11.25 g bacto-agar
autoclave 45 min (liquid cycle)
put bottles on stir plate near sterile hood until handle-able
dispense 25 mL into sterile 100 mm Petri plates