Accurate Chem YSRTmcADg YSRTMCA77G
Clone YL 1/2
Antibody as purchased (2019) was diluted 1:1 with glycerol
As purchased, should be diluted 1:200; glycerol-diluted AB should be diluted 1:100 with PBS-Tw-Az-BSA
Each tube has 4 µL antibody
Add:
400 µL PBS-Tw-Az-BSA
=404 µL, enough for 8 reactions (50 µL per coverslip, 60 min 37C)
Incubate coverslip on 50 uL antibody 1 hr 37C in humid chamber
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