Mix contents of vial with 800 mL water
pH to 6 (NaOH)
Bring to 1 L
For single batch:
Add 10g Bacto Agar
Autoclave 45 min
For small batches:
Aliquot 167 mL into 6 200 mL media bottles
Add 1.67 g Bacto Agar to each bottle
Autoclave 30 min
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