Equipment
- microfuge tubes with blue pestles
- 60C drybath
- ice
- microfuge
Chemicals:
Aliquot 600 µL each:
- CTAB buffer
- isopropanol (ice cold)
Aliquot 750 µL chloroform:isoamyl alcohol.
More than one/student for repeat extraction
CTAB extraction buffer
Recipe
| ingredient | concentration | amount per L |
|---|---|---|
| Tris pH 8 | 1M | 100 mL |
| NaCl | 8.1 % | 81.8 g |
| EDTA | 0.5 M | 40 mL |
| CTAB | 2% | 20 g |
| Beta ME | 0.2% | 2 mL |
Buy it from Fisher: Teknova C2190 CTAB Extraction Solution:
| ingredient | concentration |
|---|---|
| Tris pH 8 | 100 mM |
| NaCl | 1.4 M |
| EDTA pH 8 | 20 mM |
| CTAB | 2% |
Add Beta ME just before aliquoting: 2 µL/mL
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