crush leaf tissue in 600 µL 2X CTAB extraction buffer in microfuge tube using blue pestle
Incubate 60C 30 min. Invert 2x
Add 600 µL chloroform:isoamyl alcohol (24:1)
Centrifuge 5 min
Harvest supernate. (If it's cloudy, repeat chloroform:isoamyl step)
Add equal volume ice cold isopropyl
invert gently until 2 phases mix.
Store at -20C (at least 30 min, better overnight)
- Printer-friendly version
- Log in to post comments