Fluorescein

MW = 332.31

Excitation wavelength: 460 nm Emission wavelength: 515 nm

Absorption maxima at 493.5 and 460 nm

A = Ecl

Molar extinction coefficient of fluorescein at 485nm = 50358/mol.cm

so Absorbance 10^-5M = 0.50358

(1/100 of a 1mM solution =10^-5M)

1 mM stock in 10mM NaOH