Laney's SC high ade

Submitted by kdorfman on Sun, 01/26/2020 - 16:22

From Jeff Laney's recipe:

Per liter of medium, mix the following asceptically, and pre heat:

Component Volume (mL)
10X SC-AHLT 100
10X YNB+AS 100
10X 20% glucose 100
10X Adenine hemi sulfate 100
50X Tryptophan 20
100X Histidine HCl 10

Add to 20g agar in 667.5 mL water, autoclaved 30 minutes

Mark with one green line.