LB

Luria-Bertani broth (Miller)

LB broth (Fisher BP1426500) 500 g

25 g/L

= 20 g/800 mL (in a 1 L bottle)

Autoclave on liquid cycle 45 min

= 87.5 g/3.5L (in a 4 L flask)

Autoclave on liquid cycle 80 min

Aliquot 25 mL/tube 500 tubes (= 12.5 L)