Ampicillin

Submitted by kdorfman on Wed, 12/28/2011 - 21:02

50 mg/mL stock

(Final concentration in medium = 50 µg/mL)

Lasts up to 4 weeks in poured agar at 4C

0.5 g in 10 mL water

filter sterilize

1 mL aliquots (enough for 1 L medium)

0.4 mg/mL for QSB

Make 35 mL: 0.28 mL 50 mg/mL stock

35 mL sterile water

(filter sterilize if any doubt about sterility)

48 0.7 mL aliquots

Can also use carbenicillin

to make: mL amp solution
at this concentration: mg/mL
add: g dry ampicillin