Fern medium from powder

Carolina 156782

Mix contents of vial with 800 mL water

pH to 6 (NaOH)

Bring to 1 L

For single batch:

• Add 10g Bacto Agar

• Autoclave 45 min

For small batches:

• Aliquot 167 mL into 6 200 mL media bottles

• Add 1.67 g Bacto Agar to each bottle

• Autoclave 30 min