LB ampicilin plates for transformation
48 plates x 25 mL/plate = 1200 mL
Make 1500 mL:
25 g LB + 15 g agar /liter and 50 µg/mL ampicillin
http://wahoo.nsm.umass.edu/content/ampicillin
37.5 g LB in 1500 mL water in a 2L flask
stir to dissolve, leave stir bar in.
add 22.5 g agar
Autoclave 50 min. Include PourBoy tubing if necessary, and 2 flasks of water.
Cool till handle-able
Add 1500 µL 50 mg/mL amp
Stir
Dispense 25 mL/plate
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