Serotonin

Submitted by kdorfman on Thu, 02/23/2012 - 15:52

(Fisher A550328M050 50 mg, $34.32)

MW = 212.68

soluble up to 100 mg/mL in water

Working Concentration = 10^-5M

X M mM µM mg/mL
1X 10^-5 0.01 10 0.0021268
10X 10^-4 0.1 100 0.021268
100X 10^-3 1 1000 0.21268
1000X 10^-2 10 2.1268

1.4 mL 1000X stock

=10 mM

3 mg (use the mg scale in 266A)

1.41 mL 1X ringers or sea water

12 mL 10X solution for students to make dilutions

0.12 mL 1000X stock

11.88 mL 1X Ringers or sea water

aliquot 2 mL into 5 tubes

15 mL 1X working concentration for student use

0.015 mL 1000x stock

15 mL Ringers or sea water

aliquot 1 mL into 14 tubes