Water recipe

Submitted by kdorfman on Fri, 03/02/2012 - 15:35

Artificial Pond Water for Mussels

from http://www.biolbull.org/cgi/reprint/181/1/175.pdf

Use for partial water changes in mussel "aquaria"

Salt 1X (mM) 10X (mM) 100X (mM)
CaCl2 0.4 4 40
KCl 0.05 0.5 5
NaCl 0.5 5 50
NaHCO3 0.2 2 20

pH 7

100 X from stock solutions

80 mL aliquots to dilute to 8 L in carboy.

3 boxes changed daily for 3 weeks

Stock solution ci (M) cf (M) 80 800 1280 1600 final vol (mL)
NaCl 5 0.05 0.8 8 12.8 16 mL
KCl 1 0.005 0.4 4 6.4 8 mL
CaCl2 1 0.04 3.2 32 51.2 64 mL
NaHCO3 1 1 0.02 1.6 16 25.6 32 mL
OR dry NaHCO3 MW=84 0.02 0.134 1.344 2.15 2.688 g

pH 7

Filter sterilize (NaHCO3 may not be stable during autoclaving)

  1. make fresh; NaHCO3 is only stable for a couple of weeks. OOPS CANNOT GET THIS MUCH INTO SOLUTION! ↩︎