First thing, the morning before:
- Pick a colony
- Grow in 3 mL LB-Kan
Last thing, the day before: 100 µL culture into 40 mL (each w/ Kan)
- LB
- Lac 0.25 M
- 20 mL LB
- 20 mL Lac 0.5 M
- IPTG 0.1mM
- 20 mL LB
- 20 mL 0.2 mM IPTG
- Glu 0.25 M
- 20 mL LB
- 20 mL 0.5 M glu
- Gal 0.25 M
- 20 mL LB
- 20 mL 0.5 mL gal
- Mal 0.25 M
- 20 mL LB
- 20 mL 0.5 M mal
- IPTG 0.1 mM + glu 0.25 M
- 20 mL 0.2 mM IPTG
- 20 mL 0.5 M glu
- IPTG 0.1 mM + mal 0.125 M
- 20 mL 0.2 mM IPTG
- 10 mL LB
- 10 mL 0.5 M mal
- Lac 0.25 M + glu 0.25 M
- 20 mL 0.5M lac
- 20 mL 0.5 M glu
- Lac 0.25 M + mal 0.125 M
- 20 mL 0.5M lac
- 10 mL LB
- 10 mL 0.5 M mal
- Gal 0.25 M + glu 0.25 M
- 20 mL 0.5 M gal
- 20 mL 0.5 M glu
- Gal 0.25 M + mal 0.125 M
- 20 mL 0.5 M gal
- 10 mL LB
- 10 mL 0.5 M mal
Morning of:
- Aliquot ~4 mL each culture into plastic tubes.
- One set per 4 tables.
- ? save for next lab in fridge?
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