Lab 5: mitosis

Submitted by kdorfman on Mon, 07/30/2012 - 20:18

10 slides DAPI + tubulin

2 MatTeks per group each:

  • LLCPK with GFP tubulin
  • LLCPK parentals

NUC-Blue for staining chromosomes in live cells (Invitrogen R37605 or Fisher NC0291762), 1 bottle per station

  • Remove from culture dish after ~10 minutes. Replace stain medium with fresh medium. Otherwise it gets brighter and brighter as movie goes on

Non-CO2 medium