| 1/31 |
microscopy boot camp |
7 fixed, stained slides |
| 3/30 |
introduction to cultured cell model; Demo of sterile technique, plating etc |
3 flasks of LLCs to split & plate on MatTeks; 6 MatTeks live LL-GFP-alpha tubulin/red myosin cells to view |
| 4/4 |
Learn to stain cells; |
10 paraGlut fixed coverslips for staining |
| 4/4 |
Nucleofect (pat has siRNA) |
3 fresh flasks for nucleofection, plated Tuesday with 10^6 cells; 6 coverslips of live cells for fixing |
| 4/6 |
fix the siRNA cells |
no cells; fixative |
|
look at stained cells from 4/4 |
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look at stained organelles |
use the stained slides bioimaging |
| 4/11 |
mitosis. |
12 Live GFP tubulin cells in dishes, fixed llcpk, microtubule stain, for mitotic index, fixed at 24 and 48 hr |
| 4/13 |
mitosis: inhibitors |
12 live GFP tub in dishes, 12 live parental on coverslips for treat, fix |
| 4/18 |
quantify data from 4/13; live cell + inhibitor experiment |
Live cells in Matteks, plated Monday |
| 4/20 |
presentation for module |
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