General protocol:
Stock Solution: Add 1.5 mL MeOH to vial (~6.6µM)
Aliquots
- 5 µL stock per 0.5 mL tube
- Label: Add 200 µL PBS (or PBS 1% BSA), use 50µL per coverslip
Fix cells with formaldehyde
Procedure:
- 50 uL phalloidin on parafilm in humid chamber
- coverslip cell side down onto drop
- 15 - 20 minutes at room temp
- rinse repeatedly in PBS-Tween-azide
- blot the corner on a kimwipe
- Mount on a drop of mounting medium on a slide
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