Click-iT® Plus EdU Imaging Kits

Submitted by kdorfman on Mon, 08/12/2019 - 18:55

Materials required but not in kit:

Stock Solutions

Component conc dilute with
A (EdU) 10 mM 2 mL DMSO (C) or buffer
B (Alexa Fluor® picolyl azide)
D (reaction buffer) 10X
E Cu protectant
F (buffer additive)
G (Hoechst® 33342)

Per tube, need 200uL

To make mL Click-iT cocktail
add: uL D (reaction buffer)
add: uL Copper protectant (E)
add: uL Alexa Fluor (B)
and: uL F (buffer additive)