Home-made

Submitted by kdorfman on Mon, 08/27/2018 - 21:13

6X Home-made loading dye:

  • 30% glycerol
  • 0.3% Bromphenol blue
  • 0.3% xylene cyanol

plus SYBR-Safe at 0.5 uL/1mL

1 uL loading dye stains 5 uL of DNA sample.

SYBR safe works at 1/10,000

need 5/10,000 uL for 5 uL of sample

so need 5/10,000 uL SYBR safe for each uL of loading dye.

= 5/10 uL for each mL

NOTE Fisher Tritrack loading dye (FERR1161) composition:

  • 0.03% bromophenol blue
  • 0.03% xylene cyanol
  • 0.15% orange g
  • 60% glycerol
  • 10 mM Tris, pH 7.6
  • 60 mM EDTA

One tenth as much dye, twice as much glycerol.