2.1: Counting bacteria

Counting Bacteria

Question of the day: What concentration has the stock culture?

Materials

• Clean clear 96 well plate
• Sterile glass beads
• Sterile microfuge tubes
• Ice buckets (fill with ice at beginning of period)
• 8 LB plates per pair
• ice cold LB broth
• ~4 mL E. coli in stasis - ice cold
• spray bottle of 70% EtOH to clean tables
• hemocytometer

Program plate reader:

• Bug_OD
• A1-A12, B1-B12

Bio-hazard waste bin for

• multi-well plates
• agar plates after counting
• little tubes of E. coli
• hemocytometers