Bio 284

Bio 284 kdorfman Fri, 12/02/2022 - 16:18

Genetics lab

  • 4 sections Fall
  • 2 sections Spring

Fast Plant Seeds

Fast Plant Seeds kdorfman Fri, 12/02/2022 - 16:39

Order from Carolina Biological Supply

Kate's shopping list. This list is public. Allow others who know your email address to view the list.

Description Item # Quantity
F1 Rosette-Dwarf, Non-Purple Stem Seed (F1 Rosette, Anthocyaninless), Pack of 200 158885 (remove from 2023 order - yellow-green worked better)
F1 Non-Purple Stem, Yellow-Green Leaf Seed (F1 Anthocyaninless, Yellow-Green), Pack of 200 158891 3

Order yeast strains at the same time.

Reagents (284)

Reagents (284) kdorfman Fri, 12/02/2022 - 16:51

Check amounts of each before the semester begins

  • TAE

  • agarose Fisher BP160-500

  • sybr safe Invitrogen S33102

  • DNA ladders

  • potting soil from greenhouse

  • Enzymes from NEB

    • One Taq (large size) M0480L (small is in NEB freezer) in orange box in 362A freezer
    • OMIT 2023 S: Proteinase K P8107S (NEB freezer)
    • Aci1 (special order)
    • MnlI (special order) (plenty for 2023)
    • BsmAI OMIT 2023 S: (special order)
  • Exo-SAP-IT Fisher 78-200-200UL (in yellow "other enzymes" box)

  • 10 mM dNTP Fisher R9012 (in RT reagents box)

  • Qiagen Taq PCR master mix 201445 (Pkg says 1007544) in original packaging in 362A freezer

Yeast Strains

Yeast Strains kdorfman Fri, 12/02/2022 - 16:21

Order from Carolina Biological Supply

Kate's shopping list. This list is public. Allow others who know your email address to view the list.

Name Description Item # Quantity
Saccharomyces cerevisiae, HA0 Strain, a, wild type 173620 1
Saccharomyces cerevisiae, HB0 Strain, alpha, wild type 173621 1
Saccharomyces cerevisiae, HA1 Strain, a, ade1 173622 1
Saccharomyces cerevisiae, HB1 Strain, alpha, ade1 173623 1
Saccharomyces cerevisiae, HA2 Strain, a, ade2 173624 1
Saccharomyces cerevisiae, HAR Strain, a, ade2 173625 1
Saccharomyces cerevisiae, HB2 Strain, alpha, ade2 173626 1
Saccharomyces cerevisiae, HBR Strain, alpha, ade2 173627 1
Saccharomyces cerevisiae, HA12 Strain, a, ade1 ade2 173628 1
Saccharomyces cerevisiae, HB12 Strain, alpha, ade1 ade2 173629 1

Order Fast Plant seeds at the same time

Yeast plates (284)

Yeast plates (284) kdorfman Fri, 12/02/2022 - 16:19

Need for fall semester (4 sections):

Medium size (mm) number
YED 100 300
MV 100 150
MVADE 100 70
YEKAc 60 60

Half as many for spring