Absorbance & Fluorescence of Fluorescein, part 2
Unknowns
One too dilute to measure with abs (e.g., 0.0003mM; A3x10^-7M = 0.015)
One too concentrated to measure with Fl (e.g., above 0.01mM)
One too concentrated for either, e.g., 0.12 mM
Tell instructors the Abs and Fl of each, w/gain adjusted for the 1 mM solution
Give students:
~6 mL 10mM NaOH
~1 mL 1mM fluorescein. (Make sure Abs is correct)
Make dilution series
Check programs for correct wells.
Get a laptop for the TA to demonstrate stupid Excel tricks.
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