Supplies

Submitted by kdorfman on Wed, 01/01/2014 - 18:37

From Carolina: 131324 Chaos (Pelomyxa) carolinensis, Living $7.30

A simple mass culture of the amoeba Chaos carolinense:

http://protistology.ifmo.ru/num4_2/tan.pdf

(attached below)

Culturing Chaos is not really so simple. Order repeatedly?


131558 Paramecium multimicronucleatum, Conjugating, Living $14.60

130871 Paramecium multimicronucleatum Demoslide Set $13.50


Dishes

Amoebae must have tissue culture treated bottom to attach to.

If you use a culture flask, you can't pick up the amoebae.

Culture flask (Krackeler 40-229331 Celltreat Polystyrene Tissue culture flask 50mL Triangular TC-treated 25cm2 Angled Vent $103.00/200)

If you use culture dishes (Krackeler 40-229670 Tissue culture dish with lid Polystyrene 8mL 70 x 15mm 36.3cm2 With gripping ring $115.00/500), you can more easily see and handle them, but you risk spilling.

Bigger dishes for mass cultivation Krackeler 40-229620 Tissue culture dish with lid Polystyrene 16mL 100 x 15mm 55cm2 With gripping ring $103.00/300