Culturing Paramecium multimicronucleatum to feed Chaos
Carolina 130871 Paramecium multimicronucleatum Demoslide Set $13.50
Make medium from Carolina concentrate 132350 Liquid Protozoan Nutrient Concentrate, Culture Medium, Makes 1 gal, 100 mL $11.00
1 mL LPNC, 9 mL sterile water, 1 sterile wheat seed in a 25 mm2 vented culture flask.
1 mL P. m. culture.
20C in the dark.
- 10 10µL drops of culture
- on a clean (wipe it first!) slide
- count with dissecting scope and side illumination.
Initial concentration ~40/mL