2022

2022 kdorfman Mon, 08/29/2022 - 16:05
Lab Topic Date Date
lab 1.1 serial dilutions Tu 9/6 Th 9/8
lab 1.2 fluorescein absorbance Tu 9/13 Th 9/15
lab 1.3
lab 2.1		 Fl data
miniprep		 Tu 9/20 Tr 9/22
lab 2.2 restriction digest Tu 9/27 Th 9/29
lab 2.3 digest analysis Tu 10/04 Th 10/06
lab 3.1 counting bacteria Tu 10/11 Th 10/13
lab 3.2 bacterial growth Tu 10/18 Th 10/20
lab 4.1
lab 4.2		 qualitative and quantitative lac operon Tu10/25 Th 10/27
lab 4.3 lac operon data Tu 11/01 Th 11/03
lab 5.1 projects Tu 11/08 Th 11/10
lab 5.2 projects Tu 11/15 Tu 11/17
lab 5.3 projects Tu 11/29 Th 12/01
lab 5.4 presentations Tu 12/06 Th 12/08

5.1 2022

5.1 2022 kdorfman Tue, 11/01/2022 - 19:42

TUESDAY

Group 1

Replica plates, hand before and after treatment with:

  • 48 LB plates
  • antibacterial soap
  • regular soap
  • bleach (0.005%)
  • hand sanitizer
  • velvets
  • stamp disks

Group 2

Zone of inhibition

  • 10 LB plates
  • ON culture (RP 437)
  • filter disks
  • sterile forceps
  • miscellaneous poisons

Group 3

E. coli growth rate in min medium + sugars (overnight in plate reader)

  • M9
    • as is (glycerol)
    • +0.4% glucose
      • 0.4% galactose
      • 0.4 % maltose
      • 0.4 % lactose
  • growing E. coli RP437 (chemotaxis wild type)

See here for source of 0.4% sugars

Group 4

E. coli growth rate in liquid medium plus drugs in plate reader

  • E. coli culture (use the RP437 from the other group)
  • 2x LB
  • caffeine
  • EDTA
  • Ethanol

Group 5 E. coli growth rate at different pH

  • growing culture
  • 2x LB
  • PBS? Tris? buffered at different pH
  • Make LB in 10 mM Tris at pH 5.5, 6, 6.5, 7, 7.5, 8, 8.5, in glass tubes
    • Mix 1 M tris + Tris base to each pH,
    • 100 uL + 9.9 mL water in glass tube or vial
    • 0.25 g LB
    • autoclave

Group 6 Incubate E. coli in different concentrations of ethanol, then plate for colony counts

  • 2x LB
  • E. coli in liquid culture (RP 347)
  • ethanol
  • 50 plates

THURSDAY

Group 1

  • 4 types of mouthwash (60 mL each) student supplied

  • sterile swabs

  • 24 LB plates

Group 2

Frat floors

  • swabs

  • 24 plates

  • 4 types of disinfectants student supplied

Group 3

  • cells

  • 6 plates

  • sterile disks

  • sterile beads

  • disinfectants (student or Laura supplied)

  • sterile forceps

Group 4

Plate reader assay

  • GFP-lac cells

  • LB antibiotic liquid culture

  • LB-lac

  • LB- glu

  • LB-lac-glu

  • LB-mal

  • LB-lac=mal

  • LB

Group 5

OD plate reader experiment. (same as Tuesday Group 4)

  • 2xLB
  • caffeine
  • alcohol
  • EDTA

Group 6

Plat reader: Lac-GFP cells

  • LB

  • LB-sucrose

  • LB-lac

  • LB-glu