5.1 2022

TUESDAY

Group 1

Replica plates, hand before and after treatment with:

• 48 LB plates
• antibacterial soap
• regular soap
• bleach (0.005%)
• hand sanitizer
• velvets
• stamp disks

Group 2

Zone of inhibition

• 10 LB plates
• ON culture (RP 437)
• filter disks
• sterile forceps
• miscellaneous poisons

Group 3

E. coli growth rate in min medium + sugars (overnight in plate reader)

• M9
  • as is (glycerol)
  • +0.4% glucose
  • • 0.4% galactose
  • • 0.4 % maltose
  • • 0.4 % lactose
• growing E. coli RP437 (chemotaxis wild type)

See here for source of 0.4% sugars

Group 4

E. coli growth rate in liquid medium plus drugs in plate reader

• E. coli culture (use the RP437 from the other group)
• 2x LB
• caffeine
• EDTA
• Ethanol

Group 5 E. coli growth rate at different pH

• growing culture
• 2x LB
• PBS? Tris? buffered at different pH
• Make LB in 10 mM Tris at pH 5.5, 6, 6.5, 7, 7.5, 8, 8.5, in glass tubes
  • Mix 1 M tris + Tris base to each pH,
  • 100 uL + 9.9 mL water in glass tube or vial
  • 0.25 g LB
  • autoclave

Group 6 Incubate E. coli in different concentrations of ethanol, then plate for colony counts

• 2x LB
• E. coli in liquid culture (RP 347)
• ethanol
• 50 plates

THURSDAY

Group 1

• 4 types of mouthwash (60 mL each) student supplied

• sterile swabs

• 24 LB plates

---

Group 2

Frat floors

• swabs

• 24 plates

• 4 types of disinfectants student supplied

Group 3

• cells

• 6 plates

• sterile disks

• sterile beads

• disinfectants (student or Laura supplied)

• sterile forceps

Group 4

Plate reader assay

• GFP-lac cells

• LB antibiotic liquid culture

• LB-lac

• LB- glu

• LB-lac-glu

• LB-mal

• LB-lac=mal

• LB

Group 5

OD plate reader experiment. (same as Tuesday Group 4)

• 2xLB
• caffeine
• alcohol
• EDTA

Group 6

Plat reader: Lac-GFP cells

• LB

• LB-sucrose

• LB-lac

• LB-glu