Lab 3.1 prep

10/7 & 10/12 2021

Wet materials:

• LB plates, 8 per pair = 96/section
• LB broth (cold) ~10 mL/pair
• E. coli at stasis: 3 mL aliquots in round bottom tubes, in fridge, 1 per pair = 36 mL
• ice in small container

Equipment

• hemocytometers
• glass beads
• beaker for used beads at each table
• tips beaker
• liquid waste beaker
• microfuge tubes & rack
• marker
• clear 96 well plate
• pipetters & tips

Plate reader program

• BUG_OD
• 1st 2 rows