10X PBS from stock solutions
from Sigma ready-made 1X:
M (1X) | M (10X) | ingredient |
---|---|---|
0.01 | 0.10 | Na-K Phosphate |
0.138 | 1.38 | NaCl |
0.0027 | 0.027 | KCl |
pH to 7.3 with NaOH
sterilize
OR
To match Sigma recipe:
Make 1M Na2HPO4 (base); 1 M KH2PO4 (acid)
Mix to pH 7.3
(should be ~38.25 mL Na2HPO4, 11.5 mL KH2PO4) from the Sigma Buffer Reference Center
pH | mL Na Phos dibasic | mL Na Phos monobasic |
---|---|---|
7.2 | 36.0 | 14.0 |
7.4 | 40.5 | 9.5 |
Then
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