Lab 4.2 prep

Submitted by kdorfman on Wed, 08/25/2021 - 20:47

10/28 & 11/2 2021

Make 12 tubes of 12 media, 5 mL each.

6 sets per day.

Day before:

  • make LB-glu (mix 20% glucose from genetics 1:1 with LB if necessary)
  • Start overnight culture in LB-glu

Day of:

  • Borrow Biochem multi-channel pipet plus small tips. Dial down to 5 uL
  • Subculture into LB glu again
  • Just before use, wash with LB 3x
  • Resuspend in 10 mL LB
  • Need OD = 0.02 in the wells
    • Need 10 mL to put in sterile petri dish to fill pipet
    • Need an OD that doesn't appreciably raise the OD of 205 uL LB (OD =~0.2)
    • Aim for blank corrected OD of 0.8
    • Dilute as necessary