Lab 5.1 prep

11/18 & 11/30 2021

11/18 Thursday

Group 1: Botros et al.

• Zone of inhibition
• E. coli Addgene 20972 from molecular biology experiment
• LB Amp 25 mL
• alcohol (ethanol or isopropanol?)
• disinfectant??
• 20 LB amp plates for zone of inhibition
• sterile discs
• sterile forceps

Group 2 (Vengalil et al.):

• E. coli Addgene 20972 from molecular biology experiment grown in LB-amp
• LB amp salt-free
• LB amp 2M NaCl
  • add 2.922 g NaCl per 25 mL salt-free LB
• LB amp 2M KCl
  • add 14.9 g KCl per 100 mL LB
• 20 LB amp plates

Group 3 (Long, et al.):

• 40 LB plates (no antibiotic)
• Replica plate materials
• Kid's hand sanitizer
• room swipe access (need names and Spire IDs)

Group 4 ( Lee, et al.):

• E. coli GFP culture in LB Kan
• LB Kan ~25 mL
• LB-glu Kan ~15 mL
• 96 well black plate, shared with group 5
• rewrite script for only the rows they use

Group 5 (Creto, et al.)

• E coli GFP culture in LB Kan
• LB Kan ~25 mL
• LB-glu Kan ~15 mL
• LB-lac Kan ~15 mL
• LB-gal Kan ~15 mL
• 96 well black plate, shared with group 4
• rewrite script for only the rows they use

11/30 TUESDAY

McKnight, Carson, Matfes, Berke

• change pH of LB
• GFP expression
• plate reader

Bowen, Howall, Abraham, Wald

• fertilizer - N-based vs P-based.
• Nitrogen vs phosphorus fertilizer
• LB,
• sterile tubes,
• plates
• Culture (non-GFP?)

Boyd, Ebian, Melusen, Manning

• pH again

Cloutier, Dawiczyk, Tan, Joyce

• mouthwash
• plate reader
• culture (non-GFP?)
• Liquid LB

Alexander, Ferriera, Beinstein, Birnbach

• comparison of various substances at killing E. coli
• LB broth and plates
• E coli culture (not GFP?)

Crotty, Galligan, Horrigan, Jansen

• NaCl
• KCl
• plate reader overnight
• agar plates