Mussel saline

Submitted by kdorfman on Mon, 01/16/2012 - 15:06

Mussel Ringers

from http://www.biolbull.org/cgi/reprint/181/1/175.pdf

  • 5 mM NaCl
  • 0.5 mM KCl
  • 5 mM CaCl2
  • 5 mM NaHCO3
  • 5 mM Na2SO4

1X Ringers from 10X stock

100 mL 10X stock per L final volume

Ringers needed for

  • diluting drugs
  • keeping dissected mussels moist

10X Ringers from stock solutions

Stock solution ci (M) cf (M) 50 75 100 200 final vol (mL)
NaCl 5 0.05 0.5 0.75 1 2 mL
KCl 1 0.005 0.25 0.375 0.5 1 mL
CaCl2 1 0.05 2.5 3.75 5 10 mL
Na2SO4 1 0.05 2.5 3.75 5 10 mL
NaHCO3 1 1 0.05 2.5 3.75 5 10 mL
OR dry NaHCO3 MW=84 0.05 2.1 3.15 4.2 8.4 g

pH 7.8

Filter sterilize (NaHCO3 may not be stable during autoclaving)

  1. make fresh - it doesn't keep. ↩︎