- Put medium into dishes
- MatTek dishes for observations of live cells
- coverslip in a 35 mm dish for fixation and mounting
- Let dishes equilibrate in incubator
- Treat cells in flask with trypsin as for splitting
- Stop trypsinization with cold medium
- Add 1 - 4 drops of medium + cells to each dish, depending on cell density
- Check density of first dish, adjust volume for the remaining dishes
- Check after 24 hours.
- Printer-friendly version
- Log in to post comments