2014

Submitted by kdorfman on Fri, 10/03/2014 - 18:42

Mixtures needed to grow 50 mL cultures for qualitative experiment

Need an additional ~120 mL of each sugar for quantitative student experiments the next week. (24 5-mL aliquots each)

LB-sugar recipes

medium LB lac IPTG glu gal mal
a 50
b 25 25
c 25 25
d 25 25
e 25 25
f 25 25
g 25 25
h 12.5 25 12.5
i 25 25
j 12.5 25 12.5
k 25 25
l 12.5 25 12.5
Totals 212.5 75 75 100 75 62.5

Plus at least 100 mL for student experiments

To make 25 mL for each culture (6 sets of 12 4-mL cultures):

medium LB lac IPTG glu gal mal
a 25
b 12.5 12.5
c 12.5 12.5
d 12.5 12.5
e 12.5 12.5
f 12.5 12.5
g 12.5 12.5
h 12.5 12.5 12.5
i 12.5 12.5
j 6.25 12.5 6.25
k 12.5 12.5
l 6.25 12.5 6.25
Totals 106,25 37.5 37.5 50 37.5 31.25