Live cells on MatTek dishes
8 MatTek dishes each:
B16 (do more than one set, at different concentrations)
Must be plated lightly for motility.
Thaw LLCPK-actin, B16 24 hours before plating
Plate on all cells (lightly) Monday for Wednesday lab
3t3’s must have 36-48 hours to stretch out.
If they get overgrown (especially B16s), scrape some off
Aliquot non-CO2, serum-free medium