5.2: Motility Projects

CHECK CYTOCHALASIN STOCKS FOR 2012

2012: 500 nM (250 nM final in dish) should give an effect without causing massive detachment of cells

Stock is 10 mM in DMSO.

Need 6 1 mL aliquots of 500 nM in non-CO2 medium:

• 1 µL 10 mM stock
• 20 mL medium

2011: working concentration of cytochalasin D: 1-10uM

Make 200uM stock for students to dilute with non-CO2 medium

4 groups using cytochalasin, each doing 4 dishes.

Maximum conc 10 µM, in 2 mL

vi = (10 µM * 2 mL)/200 µM = 0.1 mL

So each group would use at most 0.4mL

Stock was 10 µL of 10 mM cytochalasin D in DMSO.

Adding 450 µL of non-CO2 medium makes it 500 µL of 200 µM
cf = (10 mM * 10 µL)/500 µL = 0.2 mM = 200 µM

Frozen stock is 2.5 mM
Invitrogen PHZ1063

Make 500 µL of 0.2mM
vi = (0.2mM * 500 µL)/2.5 mM = 40 µL
Add 460 µL non-CO2 medium

40 µL aliquots. Each can make 500 µL of 200 µM for use.

Nocodazole

Taxol