Supplies

Submitted by kdorfman on Thu, 11/03/2011 - 19:35

Get coverslip-bottom dishes from Cellvis, much cheaper than MatTek.

60 mm

35 mm

29 mm

Celltreat also has them, about $2/dish:

https://www.celltreat.com/30mm-x-10mm-tissue-culture-treated-dish-15mm-…


Microscope bulbs for phase: 12V 100 W

  • Philips 77241 or
  • Osram HLX 64623

http://www.planetbulb.com/osram-eva-64623-hlx-54251/

Microscope bulbs for Zeiss:

  • 38-01-77 6V 15W
  • OQ-77Z 3800-18-1740 6V 15W or
  • WW-6W51-8 from interlight.biz

Microscope bulbs for Olympus: BLC 120V 30W S11 BA15D from interlight.biz


Nucleofection kit

Ingenio Electroporation kit, MIR50112

www.mirusbio.com


mounting medium (SouthernBiotech Dapi Fluoromount-G, Fisher OB010020)

Hoechst solution Invitrogen™ Hoechst 33342, Trihydrochloride, Trihydrate - 10 mg/mL Solution in Water Nucleic acid stain Invitrogen™ H3570 cheaper than Thermo Fisher