| day | date | topic | prep |
|---|---|---|---|
| Tu | 9/5 | Learn your microscope | 10 HeLa parentals, FITC tubulin, 568 phalloidin, DAPI |
| Th | 9/7 | Transmitted light and alignment | |
| Tu | 9/12 | Cameras and calibration | |
| Th | 9/14 | Numerical Aperture and resolution | |
| Tu | 9/19 | Fluorescence | |
| Th | 9/21 | Fluorescence photobleaching | (10 brand new triple stained HeLa slides?) Drew says no |
| Tu | 9/26 | Immunofluorescence procedure | 20 coverslips of fixed HCT116 WT cells (grown on McCoy's) (maybe half treated with a drug the day before fixation), laminA, green secondary alpha tubulin, red secondary |
| Th | 9/28 | Immunofluorescence imaging | |
| Tu | 10/3 | Live cell imaging and cell culture | Split & seed another dish, live cell staining & imaging nuc blue, mitotracker red |
| Th | 10/5 | Plasmid prep. and cell culture | Eb1 in liquid culture, Zymo miniprep |
| Tu | 10/10 | Monday schedule | (Kate transfects HCT116's with Eb1 plasmids, seeds on viewing dishes) |
| Th | 10/12 | Transfection imaging / Makeup day | image kate-transfected cells |
| Tu | 10/17 | Midterm | Mai does trial runs |
| Th | 10/19 | FLEXIBLE DAY / CURE intro | |
| Tu | 10/24 | 1st day CURE | Students fix & stain coverslips |
| Th | 10/26 | ||
| Tu | 10/30 | ||
| Th | 11/1 | ||
| Tu | 11/6 | ||
| Th | 11/8 | ||
| Tu | 11/13 | ||
| Th | 11/15 | ||
| Tu | 11/20 | ||
| Th | 11/22 | THANKSGIVING | |
| Tu | 11/28 | ||
| Th | 11/30 | Final Project due | |
| Tu | 12/5 | Neurobiology | |
| Th | 12/7 | Neurobiology |
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