Pre-semester prep:
6 slides of each cell line (B16 30, 31, 32) all with TRITC phalloidin & DAPI
3 each:
| day | date | lab | prep |
|---|---|---|---|
| Th | 9/29 | image IF slides from previous lab | cell culture demo (9 flasks for students, 18 coverslip bottom dishes for 477H, 8 for Kari |
| Tu | 10/4 | live cell observations w/ NucBlue & Mitotracker split into 1 25 cm2 flask for nucleofection |
Eb in liquid culture |
| Th | 10/6 | split again, miniprep | |
| Tu | 10/11 | nucleofection | aliquot nucleofection reagents |
| nucleofection into dish, also flask? | |||
| Th | 10/13 | live cell imaging of transfected cells | |
| Tu | 10/18 | midterm | |
| Th | 10/20 | Plan CURE projects | |
| Tu | 10/25 | IF on coverslips: 24 B16 30, 12 B16 31, 12 B16 32 | plate on M 10/24 |
| Lamin Ac (mouse) +alpha tubulin (rat), goat anti rat red, goat anti mouse green | fix in paraformaldehyde | ||
| Hec1 (mouse) + alpha tubulin (rat), goat anti rat red, goat anti mouse green | humid chambers, colored markers, DAPI, slides |
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