2021

Submitted by kdorfman on Mon, 07/26/2021 - 16:51

Class Schedule:

date day cells reagents
9/2 Th fixed HeLa H2B-GFP mcherry-alpha Tubulin 10 untreated, and 24 treated with VPA, nocodazole, trichostatin A, cytochalasin D, latrunculin
9/7 Tu
9/9 Th
9/14 Tu PS-Speck slides B&G
9/16 Th
9/21 Tu photo bleaching HeLa slides
9/23 Th B16 on coverslips Para-glut
Rat anti-tubulin
goat anti-rat
PBS-Tw-Az
9/28 Tu 1 flask B16/pair passaging
9/30 Th view dish from previous lab
split for nucleofection
mini-prep EB1
10/5 Tu
10/7 Th
10/12 Tu
10/14 Th
10/19 Tu
10/21 Th
10/26 Tu Students split cultures from Drew into 4-chambered dishes
10/28 Th
11/2 Tu Students split onto 4 coverslips, 1 flask/pair 6-well dishes, sterile coverslips
11/4 Th view NLS-GFP cells from Drew's lab, IF PFA
rabbit anti 5mC
rabbit anti-DNMT1
goat anti-rabbit green (try red next time)
11/9 Tu
11/11 Th Veteran's Day
11/16 Tu
11/18 Th
11/23 Tu Th schedule
11/25 Th Thanksgiving
11/30 Tu
12/2 Th
12/7 Tu