PS-Speck

Submitted by kdorfman on Thu, 02/01/2018 - 13:43

Fluorescent Beads

(Should also try MultiSpeck beads M 7901)

Protocol:

  • use polylysine slides so beads stick
  • 8 uL beads total (10 is too much - 5 may be enough) (can mix colors on one slide)
  • 12 uL water; mix well
  • dry on a slide warmer (or hot plate at about 50C)
  • draw a circle on the bottom of the slide around the dried material
  • put 1 drop mounting medium over dried spot (gloppy! can't pipet!)
  • coverslip
  • nail polish

Molecular Probes P7220

Code Color Ex (nm) Em (nm) filter cube label
A blue 360 440 UV
B green 505 515 B
C orange 540 560 LP (?) G?
D deep red 633 660 probably not