fluorescent marker for Golgi in live cells
Ex: 466nm Em: 536nm
Follow instructions attached: Add 150 µL sterile H2O to the 5 mg in the bottle. (Makes 0.5mM (=500 µM) in BSA)
Aliquot 10 µL, and freeze
Working concentration is 5 µM
Add 990 µL HBSS (Hank's)(0.34 mg/mL BSA) to 10 µL in tube
Makes enough for 8 groups.
Aliquot ~120 µL per group, enough to cover the small circular coverslip in the viewing dish.
- Rinse with HBSS
- Treat with ceramide solution
- incubate in cold 30 min
- rinse 3x in cold HBSS
- replace HBSS with non-CO2 medium, incubate ~20 minutes
** Minimal rinse! Very gently! Cold cells will fall off**
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