ER Tracker Red
(BODIPY™ TR Glibenclamide), for live-cell imaging Invitrogen/Life/ ThermoFisher: E34250
Ex = 587 nm Em = 615 nm
MW = 915.23
100 ug lyophilized.
To make stock solution:
- Add 110 uL DMSO to make a 1mM solution
- Make 1 uL aliquots
To use
Working concentration: ~1 uM.
Add 1 mL HBSS to 1 uL aliquot to make 1uM solution. (Use buffered saline with Ca++ and Mg++)
treat cells with warm 1 uM solution 15 - 30 min
rinse staining solution out
put non CO2 medium in dish
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