ER Tracker

ER Tracker Red

(BODIPY™ TR Glibenclamide), for live-cell imaging Invitrogen/Life/ ThermoFisher: E34250

Ex = 587 nm Em = 615 nm

MW = 915.23

100 ug lyophilized.

To make stock solution:

• Add 110 uL DMSO to make a 1mM solution
• Make 1 uL aliquots

To use

Working concentration: ~1 uM.

• Add 1 mL HBSS to 1 uL aliquot to make 1uM solution. (Use buffered saline with Ca++ and Mg++)

• treat cells with warm 1 uM solution 15 - 30 min

• rinse staining solution out

• put non CO2 medium in dish