2024 Wadsworth

Submitted by kdorfman on Tue, 01/16/2024 - 21:39

UNIT 1

Lab Day date materials
1 Th 2/1 1 slide each scope - DAPI
2 Tu 2/6 Same slide as Lab 1, micrometer
3 Th 2/8 1 slide each scope, DAPI + 1
4 Tu 2/13 Slides with DAPI, tubulin, actin, also
beads for microscope resolution
5 Th 2/15 camera resolution; slides from Lab 4
6 Tu 2/20 photobleaching. Fresh 3-color slide
Th 2/22 NO CLASS - MONDAY SCHEDULE
Tu 2/27 Presentations

UNIT 2

Lab Day date materials
1 Th 2/29 Immunofluorescence
paraglut fixed coverslips1 for tubulin, phalloidin (2 each)
materials for mounting, slide boxes
live cells on coverslip for paraglut fix
2 Tu 3/5 live cells (GFP-tubulin LL's) in MatTek dishes
mitotracker red
ER tracker red
NBD ceramide
3 Th 3/7 miscellaneous. grow some GFP E. coli
4 Tu 3/12 learn cell culture: 8 12.5 cm2 flasks of HeLa parentals
catch-up day:
12 dishes live LL-GFP-tub for ER tracker
2 fixed coverslips for phalloidin staining
student made slides from previous lab
previously made instructor slides

UNIT 3

Lab Day date materials
1 Tu 3/26 9 coverslips HeLa, 6 coverslips HeLa treated with STLC 5 uM 18 hours, stained for alpha (2ogoat-anti-rat FITC>) and gamma (2ogoat-anti-mouse TRITC) tubulin
2 Th 3/28 HeLa: 3 coverslips, 1 flask, 3 dishes
LLCPk-1 GFP alpha 10-15 dishes
taxol
nocodazole
GSK923295 (cenp inhibitor)
cyto-D
RO3306 (CDK-1 inhibitor)
3 Tu 4/2 HeLa coverslips; LLCPk alpha GFP dishes
4 Th 4/4 HeLa: 2 live, 2 coverslip; LLCPk alpha GFP: 10 live, 4 coverslip
5 Tu 4/9 HeLa: 3 live, 5 coverslips; LLCPk-1: 10 live, 3 coverslips
6 Th 4/11 HeLa: 5 coverslips, 2 dishes; LLCPk-1: 10 live
7 Tu. 4/16 HeLa: 4 coverslips; LLCPk-1: 10 live

  1. HeLa or LLCPk1 ↩︎