from dry

Submitted by kdorfman on Wed, 01/17/2018 - 14:34

DIW (pure deionized water) 800 ml

K2HPO4 (anhydrous) 0.72 g

KH2PO4 (anhydrous) 2.16 g

Difco Bacto Peptone (not Proteose Peptone) 3.2 g

Difco Bacto Agar 12.0 g

Mix by swirling (no need to dissolve). Autoclave 10 min. Cool to 45–50° in a water bath, and pour plates about ½ full.

To make plates (100 mm)
you'll need: mL water
add: g K2HPO4
add: g KH2PO4
add: g peptone (Difco Bacto Peptone, not proteose)
add: g agar
autoclave for: minutes