NM from dry

Submitted by kdorfman on Wed, 01/17/2018 - 14:34
To make plates (100 mm)
you'll need: mL water
add: g K2HPO4
add: g KH2PO4
add: g peptone (Difco Bacto Peptone, not proteose)
add: g agar
add: g dextrose
autoclave for: minutes

If required, add IPTG 0.5mM (0.5 mL 1M per L medium) to 1 mM (1 mL 1M per L medium),

and ampicillin