Materials for 1.1


  • 1 black-walled plate per pair
  • non-sterile tips
  • Mats
  • Gloves
  • Serial dilution by 5 for demonstration purposes.
    • First tube saturated
    • Last tube blank.
  • blue flashlights & yellow filters

Computers and Plate Readers

  • Check program Repeatability (Abs 485 nm; layout A1-B12)
  • Wahoo folders must be named Tues01, Tues02, ...Tues12 and Thur01, Thur02 ... Thur12, even though the user names in the Optima program are Tue01 and Thu01, etc.
  • Import program to groups
  • Check network connections (in “my computer” connect the Z drive to Wahoo as kdorfman, kd@hh3)