Taq dilution buffer

To dilute Takara Ex-Taq

Store in freezer. Won’t freeze solid. Should keep forever.

• 50 μL Tween 20

• 50 μL Nonidet P-40 (anionic detergent = NP-40)

• 10 µL 1M DTT

• 1 mL KCl (1M)

• 200 μL Tris-Cl pH 8 (1M)

• 2 μL 0.5M EDTA pH 8

• 5 mL glycerol

• 4.2 mL ddH2O

bring to final volume, filter sterilize in BSC, freeze