Luria-Bertani broth (Miller)
LB broth (Fisher BP1426500) 500 g
25 g/L
= 20 g/800 mL (in a 1 L bottle)
Autoclave on liquid cycle 45 min
= 87.5 g/3.5L (in a 4 L flask)
Autoclave on liquid cycle 80 min
Aliquot 25 mL/tube 500 tubes (= 12.5 L)
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