90922 Mueller Hinton Broth 2 from Sigma
22 grams per liter, consisting of:
- Casein acid hydrolysate 17.5
- Beef extract 3.0
- Starch 1.5
Final pH 7.3 +/- 0.2 at 25°C
Add 17 g agar per L
Make 3.5 L in a 4L flask: (yields up to 140 plates)
77 g broth mix
stir till dissolved. Leave stir bar in
59.5 g agar
autoclave 80 minutes
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