3.4 - Phire Plant kit

Phire Plant Direct PCR Kit

Fisher F-130WH

Per plant sample:

• 20 uL Dilution Buffer

Per 20 uL reaction:

• 10 uL 2X Phire Plant PCR buffer
• (primers to 0.5 uM)
• 0.4 uL Phire hot start polymerase
• 0.5 uL Plant squish supernate
• sterile water to make 20 uL